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. 2010 Sep;51(9):4407–4415. doi: 10.1167/iovs.10-5251

Figure 5.

Figure 5.

Dual IHC localization of Als2cr4 and calbindin in horizontal cells. P30 WT mice were either (A) light- or (B) dark-adapted and killed. The retinal sections were dual stained with mAb calbindin and pAb FLJ-FM. Secondary antibodies were Alexa Fluor-568 goat anti-mouse IgG (Calbindin) and Alexa Fluor-488 goat anti-rabbit IgG (Als2cr4). The nuclei were stained with TO-PRO3 iodide. Top: magnified region of the photoreceptor layer. Middle: view of the retina at ×63 magnification. Bottom: magnified region of the OPL. White arrows denote dual immunohistochemical localization of Als2cr4 with calbindin at the OPL. Magnification, ×63.