Table 1.
Band No. | NCBI Accession No. | Protein ID | Mass (kDa) | Peptides (n) | Coverage (%) |
---|---|---|---|---|---|
2* | NP_780469 | Myosin X | 233 | 18 | 9 |
2* | NP_082297 | Myosin XIV | 229 | 10 | 6 |
3 | NP_034994 | Myosin Va | 215 | 18 | 11 |
4 | NP_001034635 | Myosin VI | 146 | 18 | 18 |
5 | Q61768 | Kinesin-1 | 110 | 7 | 9 |
6 | NP_034851 | Lamin-B1 | 67 | 5 | 9 |
7* | NP_035831 | Vimentin | 54 | 34 | 59 |
7* | NP_001032901 | Als2cr4 | 45 | 6 | 16 |
8* | NP_031419 | Beta-actin | 42 | 16 | 47 |
Summary of the eight proteins isolated by immunoprecipitation with the FLJ-FM pAb and identified by mass spectrometry, their predicted molecular mass, the number of peptides identified for each protein, and the percentage of the protein covered.
Bands that showed protein grouping ambiguity.