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. 2010 May;1(5):506–515. doi: 10.1177/1947601910376192

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Figure 1. — Loss of zyxin promotes cell survival after UV irradiation. Response of mouse embryonic fibroblasts (MEFs) to 1 minute UV-C irradiation after 16 hours using (a) MTT viability assay, (b) TUNEL assay to detect DNA fragmentation, and (c) measurement of caspase3 activation. (d) Immunoblot analysis of zyxin in wild-type (wt) and zyxin-null (Zyx-/-) MEFs, with vinculin detection as a loading control. (e) Survival rate of wild-type and zyxin-null cells after UV-C irradiation as measured by MTT assay. (f) Immunoblot analysis of zyxin protein levels. (g) Survival of cells after UV-C irradiation as measured by MTT assay. Error bars represent SEM; *P < 0.01.