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. 2010 May;1(5):506–515. doi: 10.1177/1947601910376192

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Figure 6. — Zyxin’s interaction with CARP-1 is mediated by the LIM domains. (a) Immunoprecipitation of CARP-1 in complex with zyxin; IgG heavy chains are evident in the immunoprecipitation lanes. (b) Schematic representation of Zyxin and ZyxinΔLIM proteins. (c) Expression of zyxin and zyxinΔLIM proteins in transfected Zyxin (-/-) cells. (d) Co-precipitation of CARP-1 with zyxin depends on zyxin’s LIM region. (e) Yeast 2 hybrid analysis of full-length CARP-1 interaction with the indicated zyxin LIM domains. Growth of transformed yeast on minimum medium and β-galactosidase activity were evaluated.