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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 17;184(12):6807–6814. doi: 10.4049/jimmunol.1000453

Figure 3.

Figure 3

ITK−/− fetal thymic sIEL precursors exhibit the altered migration molecule expression and defective migration capability. A. Real-time RT-PCR analysis of the expression of indicated molecules in purified CD122+ and CD122− CD3+Vγ3+ cells of E16 wild type and ITK−/− fetal thymi. Data shown were obtained from three independent experiments. B. E14 or16 fetal thymocytes of ITK−/−CCR10+/EGFP and ITK+/−CCR10+/EGFP mice were analyzed for CCR10 (EGFP) expression on Vγ3+ cells. Percentages and numbers of CCR10 (EGFP)+ Vγ3+ cells were shown. Data presented is one representative from at least 6 mice of each genotype. C. In vitro migration of wild type and ITK−/− E16 fetal thymic Vγ3+ γδ T cells to S1P, CCL27 and conditioned medium of fetal skin cultures. The migration index was calculated as a ratio of numbers of Vγ3+ cells migrating into the bottom chamber in presence of attractants vs. medium only. Data shown were obtained from two independent experiments. *** P < 0.001.