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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Immunol. 2010 May 17;184(12):6807–6814. doi: 10.4049/jimmunol.1000453

Figure 4.

Figure 4

ITK−/− sIELs and their fetal thymic precursors have normal proliferation capacities. A. Similar in vivo proliferation rates of wild type and ITK−/− sIELs. Two week or one-month old mice were treated with BrdU for 9 days and then sIELs were isolated and analyzed for BrdU incorporation by flow cytometry. Data presented were means and standard deviations from three to five experiments. B-D. CFSE-labeled E16 fetal thymocytes from ITK−/−, Vav1−/− or wild type mice were stimulated with anti-γδ TCR antibody (1μg/ml, GL4) or IL-15 (50 ng/ml) for 3 days, and analyzed by flow cytometry for the proliferation of CD3+γδ T cells. One representative of three independent experiments was shown.