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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Biomaterials. 2010 Aug 13;31(32):8218–8227. doi: 10.1016/j.biomaterials.2010.07.027

Table 2.

Serum-free medium for enhanced synaptic connectivity in NB4A (500 mL)

Component Concentration Company Catalog Number
NAP2 1 ug/ml AnaSpec 61170
h BDNF1 20 ng/ml Cell Sciences CRB 600B
h GDNF1 20 ng/ml Cell Sciences CRG 400B
r CNTF1 50 ng/ml Cell Sciences CRC 401B
h CT-11 20 ng/ml Cell Sciences CRC 700B
NT-31 20 ng/ml Cell Sciences CRN 500B
NT-41 20 ng/ml Cell Sciences CRN 501B
Antibiotic/Antimycotic1 10 μl/ml Invitrogen 15240-062
aFGF1 20 ng/ml Invitrogen 13241-013
VEGF 1651 20 ng/ml Invitrogen P2654
G5 (100×)1 2 ul/ml Invitrogen 17503-012
Cholesterol2 10 ug/ml Invitrogen 12531
Laminin2 4 ug/ml Millipore 08-125
Beta-amyloid2 200 ng/ml Millipore AG966
h Tenascin-C2 200 ng/ml Millipore CC065
Recomb. ApoE22 100 ng/ml Panvera P2002
h β-NGF1 10 ng/ml R&D Systems 256-GF
SHh2 100 ng/ml R&D Systems 1314-SH
Agrin2 100 ng/ml R&D Systems 550-AG
Heparan sulfate1 80 ng/ml Sigma D9809
Vitronectin1 100 ng/ml Sigma V0132
LIF1 20 ng/ml Sigma L5158
TNF-α2 20 ng/ml Sigma T6674
PDGF2 100 ng/ml Sigma P4056
VIP2 500 ng/ml Sigma V6130
IGF-12 50 ng/ml Sigma I2656
1

component of original medium 1

2

component of original medium 2