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C/EBPδ augments nuclear import of FANCD2. HEK293 cells were transfected with Flag-tagged C/EBPδ expression constructs and treated with MMC (1 μg/mL) for 24 h. (A) Whole-cell (WE), (B) cytoplasmic (CE), and (C) nuclear (NE) extracts were analyzed for expression of FANCD2 and C/EBPδ (anti-Flag). Tubulin and H2AX are shown as loading controls. The purity of cytoplasmic versus nuclear fractions also is shown in Fig. S7.
