Fig. 5.

C/EBPδ mediates FANCD2 interaction with IPO4. HEK293 cells were transfected with Flag-tagged C/EBPδ constructs and treated with MMC as indicated. Lysates were immunoprecipitated (IP) with (A) anti-Flag antibody for C/EBPδ, (B) anti-IPO4 antibody, or (C) anti-FANCD2 antibody or respective IgG control. LE, long exposure; SE, short exposure. Western blots of input (shown in A) and immunoprecipitates were analyzed for expression of the indicated proteins. In this experiment, C/EBPδ was detected by anti-C/EBPδ antibody (BD69319; BD Biosciences). (D) Schematic (not drawn to scale) indicating how FANCD2 and IPO4 interact with different domains of C/EBPδ but not with each other (see Fig. 1A for designation of C/EBPδ domains).