Loss of function of AtTCTP results in delayed embryo development. (A) Siliques produced by WT or by heterozygous AtTCTP/tctp-1 and AtTCTP/tctp-2 plants. Arrows indicate tctp homozygous seeds. These seeds were white in color and segregated at a 3:1 ratio [χ2(3:1) = 1.03, P > 0.3 for tctp-1 and χ2(3:1) = 0.90, P > 0.3 for tctp-2]. (B) Embryos from white homozygous tctp-1 and tctp-2 seeds show delayed development compared with embryos of the same age from WT seeds. Developmental stages of WT embryo are denoted as “heart”, “torpedo,” and “cotyledons”. [Scale bars, 50 μm (heart stage) and 100 μm (torpedo and cotyledons stages).]