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. 2010 Aug 24;107(37):16384–16389. doi: 10.1073/pnas.1007926107

Fig. 2.

Fig. 2.

Embryos homozygous for tctp-1 or tctp-2 mutations can be rescued by supplying nutrients in vitro. (A) Homozygous tctp-1 and tctp-2 embryos rescued by nutrient supplements in vitro develop into adult plants. WT plant development is used as a control. Days after germination are indicated as 5 d, 10 d, 45 d, 80 d, and 95 d. [Scale bars, 500 μm (5 and 10 d) and 1 cm (45 d).] (B) Semiquantitative RT-PCR analysis showing no expression of AtTCTP in leaves of both tctp-1 and tctp-2 plants. GAPDH was used as control. (C) Western blot analysis of AtTCTP accumulation in leaves of WT, tctp-1, and tctp-2 plants using anti-AtTCTP antibody. No AtTCTP protein accumulation is observed in tctp-1 and tctp-2 plants. Red Ponceau staining of total proteins is shown as control.