Fig. 2.

GH-dependent transduction pathways analysis in Zmpste24^−/− mice. Human recombinant GH was injected i.v. in overnight-fasted Zmpste24^+/+ and Zmpste24^−/− mice. After 10 min, the mice were euthanized, and their livers were collected and flash-frozen. Liver response to GH was further analyzed by immunoblotting against major components of GH-induced signaling cascades. (A) Immunoblotting against total and phosphorylated forms of Jak2, Stat3, Stat5, and Erk1/2 in WT and Zmpste24^−/− mice livers. (B) Densitometry analysis of the phosphorylated forms of GH-related signaling transducers shown in A. At least three WT and six mutant animals were used for each assay. *P < 0.05, two-tailed t test.