Fig. 1.
Nuclear β-catenin expression in the MD mesenchyme. Membranous expression of β-catenin is observed by immunofluorescence (green) in E14.5 male and female urogenital ridges of control Amhr2-Cre/+ (A, C, E, G) in the epithelial cells but is largely nuclear in the MD mesenchyme of Amhr2-Cre/+;Ctnnb1Δ(ex3)/+ (B, D, F, H) embryos. DAPI staining is shown in blue to mark nuclei. Gonocytes cells are shown by mvh immunofluorescence (red). O, ovary; T, testis; MDM, Müllerian duct mesenchyme; MDE, Müllerian duct epithelium; WD, Wolffian duct. (Scale bars, 50 μm.)