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K5 targets HFE “high” from a post-Golgi compartment. FLAG-HFE plus empty lentiviral vector and HFE/K5 cells were metabolically labeled and subjected to primary IP using anti-β2m at different chase time points and then secondary IP using anti-MHC-I and anti-FLAG antibodies and analyzed by SDS/PAGE. The appearance of the HFE high form was disrupted by K5 expression, with smearing toward higher molecular weight forms (indicated by *). Size markers are in kilodaltons.
