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. 2010 Aug 30;107(37):16303–16308. doi: 10.1073/pnas.1005077107

Fig. 1.

Fig. 1.

Immunoprecipitation of RIG-I/RNA complexes from 24-h SeV infections and enzymatic analysis of RIG-I–associated RNA. (A) A schematic representation of the experimental procedure for characterization of RNA from RIG-I IPs. (B) Western blot analysis from RIG-I pulldowns shows the efficiency of RIG-I immunoprecipitations. (C) Immunostimulatory activity of RNA from RIG-I and control (GFP) IPs upon transfection into 293T ISRE-FF cells. (D) Enrichment of immunostimulatory RNA with RIG-I IP compared with total RNA from SeV-infected cells.