Fig. 4.

Immunoprecipitation and deep sequencing analysis of RIG-I–associated RNA from 24-h influenza virus infections. (A) Sequencing reads from RIG-I and control (GFP) IPs were mapped to the individual PR8 influenza virus genomic segments. Each peak corresponds to the beginning position of that particular read and the number of sequences starting at that position is represented on the y axis. RIG-I enrichment (blue) can be observed on all genomic segments. (B) Transfection of RIG-I–associated RNA into the ISRE-FF reporter cells shows high immunostimulatory activity of this RNA compared with the control (GFP) IP.