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. 2010 Aug 26;107(37):16113–16118. doi: 10.1073/pnas.1010580107

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Fig. 2. — Oligomerization of the kinase/RNase domain of human IRE1 in vitro. (A) Schematic of the IRE1(KR43) construct expressed in insect SF9 cells. IRE1(KR43) contains a 43-aa portion of linker, kinase (“K”) and endoribonuclease (“R”) domains of human IRE1. (B) Cooperative activation profile for IRE1(KR43) obtained using 5′-³²P-HP21. Solid curve indicates profile of IRE1(KR43) with n = 3.4; dashed curve indicates predicted activation profile with n = 2 for comparison purpose. Schematic representation of RNA substrate (HP21) used in the in vitro experiments. Arrowhead marks site of specific cleavage by human IRE1 (KR43).