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. 2010 Aug 26;107(37):16072–16077. doi: 10.1073/pnas.1006026107

Fig. 2.

Fig. 2.

The effect of ionic conditions and template topology on premature transcription termination. (A) Wt (lanes 1–6) and mutant (lanes 7–12) templates were transcribed in the presence of GTP (lanes 1–3, 7–9) or 7-deaza-GTP (lanes 4–6, 10–12) and 35 mM of added NaCl, KCl, or LiCl. PT(%), percentage of pretermination; M, marker lane. (B) In vitro transcription on SspI-linearized (lanes 1–3 and 7–9) or supercoiled (lanes 4–6, 10–12) template. A final concentration of 35 mM of the indicated ion was added, and reactions contained either GTP or 7-deaza-GTP. PT, preterminated transcript; RO lin, runoff transcript from linearized template (∼240 nts); RO sc, runoff transcripts from supercoiled template. M, marker lane.