Table 1.
Oligonucleotides used in the native gel analysis
RNA oligo | Sequence |
CSB II WT | GAAGCGGGGGAGGGGGGGUUUGGUGGAAAU |
G → A mut | GAAGCAAAAAAAAAAAAAUUUAAUGGAAAU |
295–296 mut | GAAGCGGGGGAGGGGGGGUUUGGUAAAAAU |
305–306 mut | GAAGCGGGGGAGGGAAGGUUUGGUGGAAAU |
312–313 mut | GAAGCGGAAGAGGGGGGGUUUGGUGGAAAU |
Sequences with guanines in CSB II that are predicted to have G4-forming potential are underlined and mutated nucleotides are indicated in bold font. The numbering refers to the Cambridge reference sequence (29).