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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Jul 22;30(10):1940–1948. doi: 10.1161/ATVBAHA.110.205997

Figure 1.

Figure 1

Figure 1

A) Protein level of HN in different tissues in mice: A total of 30 µg of protein from mouse heart (lane 1), skeletal muscle (lane 2), liver (lane 3) and 3 ng of synthetic RN (rodent homolog of HN) peptide (lane 5) were loaded. Higher amounts of HN protein were detected in heart compared to muscle and liver. B) Protein level of HN in different cell types in heart: 30 µg of mouse heart (lane 1), cardiomyocyte (lane 2), smooth muscle vascular cell (lane 3), human umbilical vein endothelial cells (lane 4) and 3 ng of synthetic RN peptide (lane 5) were loaded. HN levels were highest in the cardiomyocytes (*p< 0.001 vs. other cell lines). C) Protein levels of HN post MI-R: Protein extracts from left ventricle at basal (lane 1), 4 hr (lane 2), 12 hr (lane 3) and 24 hr (lane 4) post MI-R were loaded. The levels of HN increase post MI-R and persist up to 24 hrs post injury (*p < 0.01 vs. baseline).