The 1.8LHβluc reporter gene was transiently transfected into LβT2 cells along with the indicated receptor expression vector. After overnight starvation in serum-free media, the cells were treated for 24 h with hormone. The results represent the mean ± sem of at least three experiments performed in triplicate and are presented as fold induction relative to the vehicle control. *, Indicates that the hormone treatment was significantly different from the vehicle-treated control using Student’s t test. A, LβT2 cells were transiently transfected with the AR expression vector and then treated with 100 nm R1881. B, Cells were transiently transfected with the PR expression vector and then treated with 100 nm R5020. C, Cells were transiently transfected with the GR expression vector and then treated with 100 nm dexamethasone (Dex).