Table 2. Refinement statistics.
| Parameters | 5′-capped RNA | |||
| Resolution range (Å) | 49.7 – 2.9 (3.0 – 2.9) | |||
| Rfactor #,∧ (%) | 20.5 (32.8) | |||
| Rfree * ∧, (%) | 23.1 (34.4) | |||
| No of non-H atoms/chain | A | B | C | D |
| Protein | 2075 | 2056 | 2060 | 2060 |
| SAH | 26 | 26 | 26 | 26 |
| 5′-capped RNA | 204 | 204 | ||
| Glycerol | 12 | |||
| Water | 463 | |||
| Mean B-factor+ (Å2) | ||||
| Protein | 20.1 (47.9) | 20.0 (47.8) | 20.0 (48.3) | 20.0 (48.3) |
| SAH | 59.8 (53.5) | 56.8 (50.9) | 54.7 (53.0) | 55.0 (53.5) |
| 5′-capped RNA | 7.9 (87.1) | 22.8 (87.0) | ||
| Glycerol | 85.1 (78.0) | |||
| Water | 26.7 (56.9) | |||
| Rms deviations | ||||
| Bond lengths (Å) | 0.0062 | |||
| Bond angles (°) | 1.21 | |||
| Ramanchandran plot | ||||
| Most favoured (%) | 92.8 | |||
| Allowed regions (%) | 6.7 | |||
| Disallowed regions (%) | 0.5 | |||
| Overall G factor$ | 0.15 | |||
#
Rfactor = Σ ||Fobs| - |Fcalc||/Σ | Fobs|.
*Rfree was calculated with 5% of reflections excluded from the whole refinement procedure.
$
G factor is an overall measure of structure quality from PROCHECK [41]. The numbers in parentheses refers to the last (highest) resolution shell.
∧
Bound (“B”) monomers and free (“F”) monomers were tightly restrained as two separate groups.
+
Mean B-factors are shown after TLS refinement. Mean B-factors without TLS refinement are given in parenthesis for comparison.