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. 2010 Sep 17;5(9):e12837. doi: 10.1371/journal.pone.0012837

Figure 6. Vitronectin induces ruffled borders and clear zones in osteoclasts.

Figure 6

Osteoclasts were incubated for 5 hours in MEM/BSA with M-CSF (50 ng/ml), RANKL (30 ng/ml) and IL-1α (10 ng/ml) in 6-well plate wells coated with vitronectin or fibronectin (50 µg/ml), before raising into suspension with a cell scraper and preparation for TEM. A: Osteoclast incubated on vitronectin shows a central area of ruffled border (arrowhead) and a peripheral area free of organelles (‘clear zone’) (arrows). B, C: higher magnification of center (B) and lower portion (C) respectively of A, showing area of ruffled border (B) and clear zone (C); D–F: Osteoclast incubated on fibronectin shows well-spread appearance, but the undersurface lacked the membrane folds and clear zones seen in osteoclasts incubated on vitronectin. E and F are from central and lower portion of D respectively. Scale bars: A, D: 5 µm; B, C, E, F: 1 µm.