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. 2010 Sep 17;5(9):e12826. doi: 10.1371/journal.pone.0012826

Figure 7. HCV protein and RNA abundances in HuH-7 cells after infection with JFH1 virus, followed by transfection of plasmids encoding GFP and GFP-Core peptides.

Figure 7

HuH-7 cells were infected with JFH1 virus. Two days after infection cells were transfected with pGFP-Core plasmids (pGFPHCVc16-34, pGFPHCVc16-35, pGFPHCVc16-36). (A) HCV and GFP-Core fusion protein levels were examined in Western blots using anti-NS5A MAb 9E10, anti-GFP and anti-Actin antibodies. (B) HCV and actin RNA levels were analyzed in Northern blots.