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. 2010 Sep 17;5(9):e12840. doi: 10.1371/journal.pone.0012840

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Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Overnight cultures of bacterial strains grown in BHI at 26°C were harvested and whole cell lysates were separated by SDS-PAGE. The expression of rovA was detected by Western blotting using a rabbit polyclonal antibody against His-tagged RovA. For the induction of RovA expression in ΔrovA-pAraRovA, arabinose was added to the culture medium at the indicated concentration.