Figure 6. Ability of RovA to bind to promoters of lcrF and lcrG of T3SS.

The upstream regions of lcrG, yscA, lcrF and psaE were amplified by PCR and used as target DNA probes in EMSA. The [γ-32P]-labeled target DNA probes were incubated with or without increasing amounts of purified His-RovA protein (lanes 1–4). Three controls were included in each EMSA experiment as indicated: 1) non-specific probe competitor (unlabeled DNA probe containing promoter of a gene that was shown to be not affected by rovA mutation); 2) specific probe competitor (unlabeled DNA probe containing promoter region of the investigated gene); and 3) unrelated proteins (rabbit anti-F1-protein polyclonal antibody).