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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Alcohol. 2010 Aug 12;44(6):531–540. doi: 10.1016/j.alcohol.2010.06.003

Figure1. Ethanol induced ROS generation in hepatocytes.

Figure1

Primary culture of rat hepatocytes were treated with 50 mM or 100 mM ethanol in 6 well plates for 23 hr. DCF-DA (10 μM) was added to the wells. After 1 hr, the media was removed from the wells and replaced with fresh phenol free DMEM media. Fluorescent intensity of the cell was measured at 485 nm excitation and 520 nm emission in a Packard Fusion plate reader. Data represent mean ± SE (bar), n=3 experiments. Values correspond to fold increase compared to control. *p<0.001 (Ethanol compared to Control) (C=Control; E50=50 mM Ethanol; E100=100 mM Ethanol).