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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Alcohol. 2010 Aug 12;44(6):531–540. doi: 10.1016/j.alcohol.2010.06.003

Figure 2. Effect of glutathione modulators on ethanol induced histone H3K9 acetylation in hepatocytes.

Figure 2

Figure 2

Hepatocytes were pretreated with a) 10 mM NAC or b) 1 mM BSO for 1 hr followed by with or without 50 mM ethanol for 23 hr. Nuclear extracts were prepared for immunoblot analysis to detect acetylated histone H3 at lys9. Identical amounts (40 μg) of extract were subjected to 15% SDS–PAGE and transferred onto nitrocellulose membrane. H3AcK9 levels were monitored using anti-H3 AcK9 antibody and ECL detection. A western blot from a representative experiment is shown. Quantitative analysis of acetylated histone H3 was performed by densitometric analysis and is presented in histogram as mean ± SE (bar), n = 3 experiments. Values represent fold increase compared to control (C=Control; E50=50 mM Ethanol; N=10mM N-acetyl cystein; E50+N=50 mM Ethanol+10 mM N-acetyl cystein; B=1 mM Buthionine -S-sulfoxamine; E50+B=50 mM Ethanol+1 mM Buthionine -S-sulfoxamine). Fig. 2a) *p<0.01(E compared to C) and **p<0.01 (E50 + N compared to E50). Fig. 2b) *p<0.001 (E compared to C), **p<0.001 (E compared to E + B), *p<0.05 (C compared to B).