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. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: Alcohol. 2010 Aug 12;44(6):531–540. doi: 10.1016/j.alcohol.2010.06.003

(A) Hepatocytes were pretreated with H₂O₂ (10 or 50 μM H₂O₂) for 1 hr. Subsequently, cells were stimulated with 50 mM ethanol for 23 hr. Acetylated histone H3 at lys9 was measured as described in the Materials and Methods and Fig. 2. Values represent the mean of 3 separate experiments, with the ±SE indicated by vertical lines. Values represent as fold increase compared to control (C=Control; E50=50 mM Ethanol; H10=10 μM Hydrogen peroxide; H50=50 μM Hydrogen peroxide; E50+ H10=50 mM Ethanol+10 μM Hydrogen peroxide; E50+ H50=50 mM Ethanol+50 μM Hydrogen peroxide). *p<0.001 (C compared to E50 and H50), **p<0.001 (E compared to E50+ H50). (B) Hepatocytes were treated with 50 μM H₂O₂ for 24 hrs in the presence or absence of NAC (10 mM) and the levels of H3AcK9 or ADH1 mRNA were monitored.

(A) Hepatocytes were pretreated with H₂O₂ (10 or 50 μM H₂O₂) for 1 hr. Subsequently, cells were stimulated with 50 mM ethanol for 23 hr. Acetylated histone H3 at lys9 was measured as described in the Materials and Methods and Fig. 2. Values represent the mean of 3 separate experiments, with the ±SE indicated by vertical lines. Values represent as fold increase compared to control (C=Control; E50=50 mM Ethanol; H10=10 μM Hydrogen peroxide; H50=50 μM Hydrogen peroxide; E50+ H10=50 mM Ethanol+10 μM Hydrogen peroxide; E50+ H50=50 mM Ethanol+50 μM Hydrogen peroxide). *p<0.001 (C compared to E50 and H50), **p<0.001 (E compared to E50+ H50). (B) Hepatocytes were treated with 50 μM H₂O₂ for 24 hrs in the presence or absence of NAC (10 mM) and the levels of H3AcK9 or ADH1 mRNA were monitored.