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. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: Alcohol. 2010 Aug 12;44(6):531–540. doi: 10.1016/j.alcohol.2010.06.003

Hepatocytes were pretreated with a) rotenone (0.50 μM or 1 μM) and b) 0.025 mM antimycin for 1 hr. Then cells were treated with 50 mM ethanol for 23 hr. Equal amounts (40 μg) of nuclear extract were used for Western blot analysis to detect acetylated histone H3 at lys9. Quantitative analysis of acetylated histone H3 was performed by densitometric analysis and is presented as mean ± SE (bar), n = 3 experiments. A representative blot is also shown. Values represent as fold increase compared to control (C=Control; E50=50 mM Ethanol; Rt0.5=0.5 μM Rotenone; Rt1=1 μM Rotenone; E50+ Rt0.5=50 mM Ethanol+0.5 μM Rotenone; E50+ Rt1=50 mM Ethanol+1 μM Rotenone; An=0.025 mM Antimycin; E50+An=50 mM Ethanol+0.025 mM Antimycin) a) *p<0.05 (C compared to E and Rt1), **p<0.01 (E compared to E+ Rt1)

Hepatocytes were pretreated with a) rotenone (0.50 μM or 1 μM) and b) 0.025 mM antimycin for 1 hr. Then cells were treated with 50 mM ethanol for 23 hr. Equal amounts (40 μg) of nuclear extract were used for Western blot analysis to detect acetylated histone H3 at lys9. Quantitative analysis of acetylated histone H3 was performed by densitometric analysis and is presented as mean ± SE (bar), n = 3 experiments. A representative blot is also shown. Values represent as fold increase compared to control (C=Control; E50=50 mM Ethanol; Rt0.5=0.5 μM Rotenone; Rt1=1 μM Rotenone; E50+ Rt0.5=50 mM Ethanol+0.5 μM Rotenone; E50+ Rt1=50 mM Ethanol+1 μM Rotenone; An=0.025 mM Antimycin; E50+An=50 mM Ethanol+0.025 mM Antimycin) a) *p<0.05 (C compared to E and Rt1), **p<0.01 (E compared to E+ Rt1)