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. Author manuscript; available in PMC: 2011 Sep 17.
Published in final edited form as: Circ Res. 2010 Jul 22;107(6):737–746. doi: 10.1161/CIRCRESAHA.109.215715

Figure 3. Confirmation of genes upregulated by OxPAPC.

Figure 3

Bone marrow-derived macrophages were stimulated with 10U/ml IFNγ and 1μg/ml LPS (M1), 10ng/ml IL-4 (M2), indicated concentrations of OxPAPC (Mox) or medium only (co). RNA was isolated after 3 hours of stimulation, and reverse transcribed. RT-PCR was performed and expression of iNOS and arginase 1 (A), HO-1 (B) or VEGF (D) was performed. n=4, results are shown as mean average ±SD. p<0.05. For flow cytometric analysis (C), cells were stimulated with 10U/ml IFNγ and 1μg/ml LPS (M1), 10ng/ml IL-4 (M2), OxPAPC (Mox), medium only (co) or with IL-10 as positive control for 18 hours. Macrophages were fixed and stained for HO-1 using an Alexa-488 labeled rabbit anti HO-1 antibody.