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. Author manuscript; available in PMC: 2011 Sep 17.
Published in final edited form as: Circ Res. 2010 Jul 22;107(6):737–746. doi: 10.1161/CIRCRESAHA.109.215715

Figure 4. Oxidized phospholipids skew macrophage phenotypes toward Mox.

Figure 4

Bone marrow-derived macrophages were polarized with either 10U/ml IFNγ and 1μg/ml LPS (M1) or 10ng/ml IL-4 (M2), or control medium only (M0) followed stimulation with either 10 μg/ml PAPC or indicated doses of OxPAPC. RNA was isolated after 3 hours, and gene expression was analyzed using RT-PCR. Typical M1-gene expression (A), as well as arginase-1 expression, a marker for M2 (B) was significantly reduced when stimulated with OxPAPC. HO-1 expression was significantly induced in M0, M1, and M2 macrophages, when stimulated with OxPAPC (C). n=4, results are shown as mean average ±SD, fold over M0; *p<0.05.