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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Clin Immunol. 2010 Jul 14;137(1):74–80. doi: 10.1016/j.clim.2010.06.011

Figure 5. Assessing neutrophil apoptosis.

Figure 5

Isolated neutrophils from normal controls (solid bars, n=4) or XLA subjects (open bars, n=4) were cultured for 48 hours with CL097 (2.5μg/mL), LPS (100ng/mL) or GM-CSF (20ng/mL), and induction of apoptosis assessed by Annexin V, (5a) or late apoptosis PI, (5b). Data is represented as a percentage of Annexin or PI positive cells. P values denoted represent two-tailed t-test comparison of each treatment to the untreated control neutrophils. No differences were seen between normal and XLA neutrophils in any treatment group. Noted significance (*p<0.05, **p<0.01, ***p<0.005) represents comparison to untreated neutrophils from same patient or control subject.