Fig. 4.
SSL7 affects binding of C5 to C5 convertases. (A,B) Complement ELISA experiments showing C3b (A) and C5b-9 (B) deposition after complement activation of the classical (5% serum), lectin (5% serum), or alternative (25% serum) pathway in the presence of 0 – 450 nM SSL7. Results are expressed relative to control-treated serum and represent mean values ± SEM of three independent experiments. (C) Binding of C5 to (C3b)2 in the presence of 10 μg/ml CRIg (Complement Receptor of the Immunoglobulin superfamily recognizes C3b molecules), SSL7 (450 nM), or SSL7 (450 nM) and IgA (60 μg/ml). Results represent mean values ± SEM of two independent experiments.