Table 3.
Effect of α-tocopherol supplementation to embryo culture medium on development of preimplantation sheep embryos cultured in 5% oxygen environment
α-tocopherol conc. (µM) | No. of oocytes inseminated | Cleavage (%) | Morula (%) | Blastocyst (%) | No. of blastocysts evaluated | Total cell number |
---|---|---|---|---|---|---|
0 | 373 | 65.49 ± 1.27a | 33.11 ± 1.93ab | 10.54 ± 0.86ab | 17 | 88.33 ± 2.82a |
50 | 386 | 66.36 ± 1.26a | 34.82 ± 1.97ab | 11.80 ± 0.66ab | 18 | 91.50 ± 4.26a |
100 | 368 | 68.55 ± 1.33a | 36.47 ± 2.43ab | 12.69 ± 1.84ab | 16 | 92.67 ± 4.28a |
200 | 377 | 68.91 ± 0.81a | 39.20 ± 1.90a | 14.54 ± 0.64a | 19 | 94.17 ± 4.90a |
400 | 391 | 64.70 ± 2.14a | 34.44 ± 2.45ab | 10.39 ± 0.98ab | 17 | 90.33 ± 3.67a |
500 | 369 | 64.34 ± 0.54a | 29.19 ± 1.76b | 9.12 ± 0.47b | 19 | 88.50 ± 4.09a |
Values are listed as mean±SEM; means in the same column with different superscripts were significantly different