. 2010 May 8;27(8):483–490. doi: 10.1007/s10815-010-9430-7

Table 4.

Effect of α-tocopherol supplementation to embryo culture medium on development of preimplantation sheep embryos cultured in 20% oxygen environment

α-tocopherol conc. (µM)	No. of oocytes inseminated	Cleavage (%)	Morula (%)	Blastocyst (%)	No. of blastocysts evaluated	Total cell number
0	369	57.17 ± 1.26^b	24.08 ± 2.14^b	8.24 ± 0.69^c	18	88.00 ± 4.42^b
50	394	57.94 ± 1.16^ab	25.66 ± 1.36^ab	11.49 ± 1.13^bc	17	95.33 ± 4.77^ab
100	386	59.34 ± 0.96^ab	28.33 ± 1.03^ab	15.41 ± 0.87^ab	20	101.33 ± 2.36^ab
200	378	62.31 ± 1.09^a	32.12 ± 1.93^a	17.60 ± 1.73^a	19	111.33 ± 3.14^a
400	365	61.56 ± 1.20^ab	28.07 ± 1.20^ab	14.05 ± 1.28^ab	16	101.50 ± 4.01^ab
500	372	58.08 ± 1.25^ab	24.70 ± 2.27^b	12.49 ± 0.56^bc	16	94.50 ± 3.18^b

Values are listed as mean±SEM; means in the same column with different superscripts were significantly different