Table 5.
Summary of plasmids used
| Plasmid name | Derived from plasmid | Recombinant protein expressed | F1 origin | cer fragment | Kanamycin resistance gene | ccdA gene |
|---|---|---|---|---|---|---|
| pET28c | - | + | - | + | - | |
| pM1800 | pET28c | - | - | + | + | - |
| pMH.P3.1 | pET28c | AlpA | + | - | + | - |
| pM1816 | pM1800 | rEPA | - | + | + | - |
| pSP301 | pM1800 | - | - | + | Rcb | + |
| pSP1 | pM1816 | rEPA | - | + | - | + |
| pSP2 | pSP301 | - | - | - | Rcb | + |
| pSP3 | pSP2 | AlpA | - | - | Rcb | + |
| pSP4 | pSP2 | rEPA | - | - | Rcb | + |
| pSP5 | pSP301 | AlpA | - | + | Rcb | + |
| pSP6 | pSP301 | rEPA | - | + | Rcb | + |
-: absent; +: present; Rcb: kanamycin resistance gene is deleted through homologous recombination