Figure 4.

Effects of p13 on T-cell proliferation. Shown is a proliferation analysis of Jurkat T cells transduced with a lentiviral vector expressing p13 or with a control empty vector. Cells were labelled with the cell membrane fluorescent probe PKH26 (sigma) on day 0 and proliferation was assessed by measuring the dilution of the fluorescent signal resulting from cell division was monitored on days 2 and 6. A) Flow cytometry analysis of PKH26 staining demonstrating a higher proportion of cells of higher generation in control compared to p13-expressing cells. Colors indicate generations of cells exhibiting progressively lower intensities of PKH26 staining (Blue, generation 1= parental; orange, generation 2; green, generation 3; purple, generation 4; light blue, generation 5); arrow indicates peak fluorescence values of parental (time o) generation for the two cell lines. B) Mean proliferation indexes over time calculated from the PKH26 analyses in control vs. p13-expressing cells.