Table 1.
Assessment of IFN-γ levels at approximately 24 h after the start of schizont rupture in three independent experiments using multiple parasite strains
| Experimenta | Parasite Strain | Parasitaemia and maturity at start of experiment | Time point approx 24 h after schizont rupture | IFN-γ 1 pg/mLb | IFN-γ 2 pg/mLb | Mean IFN-γ pg/mL |
|---|---|---|---|---|---|---|
| A | Palo Alto | 4·0% mature pigmented trophozoites | 30 h | 86·1 | 128·3 | 107·2 |
| A | TM284 | 4·5% mature pigmented trophozoites | 42 h | 158·5 | 109·5 | 134·0 |
| A | R29 | 4·0% mature pigmented trophozoites | 42 h | 171·9 | 174·4 | 173·2 |
| B | Palo Alto | 2·0% bursting schizonts, 22·0% small rings | 18 h | 1459·7 | ND | 1459·7 |
| B | TM284 | 4·4% bursting schizonts, 3·8% small rings | 18 h | 3670·1 | 3370·1 | 3520·1 |
| B | Field isolate A | 5·8% schizonts | 32 h | 851·9 | 828·6 | 840·3 |
| B | Field isolate B | 5·1% schizonts | 32 h | 762·3 | 1015·6 | 889·0 |
| B | Field isolate C | 4·4% mature pigmented trophozoites | Mean of 32 and 40 h | 939·6 | 801·3 | 870·5 |
| C | Palo Alto | 1·3% large rings | 42 h | 5·0 | 38·3 | 21·7 |
| C | TM284 | 0·5% mature pigmented trophozoites | 42 hc | 5·2 | 2·5 | 3·9 |
| C | Field isolate D | 0·5% mature pigmented trophozoites | 42 hc | 70·9 | 58·4 | 64·6 |
| C | Field isolate E | 0·7% schizonts | 24 h | 65·0 | 58·2 | 61·6 |
a
Each experiment used a different single donor. Experiment A is shown in detail in Figure 4. Experiments B and C are as described in the text;
b
IFN-γ levels in culture supernatants of two replicate wells were measured at each time point by ELISA;
c
For both TM284 and Field isolate D, the final time point studied (42 h) was about 18 h after schizont rupture began. Ideally, a 48-h time point (giving data 24 h after schizont rupture) would have been optimal; however, this was not available for this experiment.