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. Author manuscript; available in PMC: 2011 Jul 16.
Published in final edited form as: ACS Chem Biol. 2010 Jul 16;5(7):667–680. doi: 10.1021/cb100054m

Fig. 4. d-Switch comparison of GST-P1 nitrosation by six NO-donors in pure form and in human cells.

Fig. 4

(A) Concentration dependent Cys47 nitrosation after incubation of GST-P1 (20 μM) with the indicated concentrations of various NO-donors at 37 °C for 30 min from d-Switch analysis. Data showing mean and s.e.m. of 3 experiments for IAN, DEA/NO and ODZ1 are linearly fit with slopes of 0.031, 0.021, and 0.0014, respectively. (B) Effect of O2 pressure on GST-P1 nitrosation at Cys47 by NO-donors (500 μM) analyzed by d-Switch. Data show mean and s.d. of 3 experiments (C) Concentration dependant Cys47 nitrosation after incubation of human neuroblastoma SH-SY5Y cells with CysNO for 20 min and analysis by d-Switch (solid line; open square); data for recombinant protein incubation with CysNO are shown for comparison (dashed line; solid square).