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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: Mol Pharm. 2010 Jun 7;7(3):664–679. doi: 10.1021/mp900223c

Fig.10. Glucuronidation of 3, 7-diHF, 3, 5-diHF and 3, 4′-diHF by 12 Human UGTs.

Fig.10

Experiments were conducted at three concentrations (2.5, 10, and 35μM). Glucuronidation of 3,7-diHF and 3′4′-diHF produced either one or two mono-glucuronide (M1 and M2) depending on which UGT was used. Glucuronidation of 3, 5-diHF only produced one metabolite at 2.5 and 10μM, and two metabolites at 35μM. The experiments were conducted at 37 °C for 1 (or 0.5) hr and the amounts of glucuronides formed were measured using UPLC. Rates of glucuronidation were calculated as nmol/min/mg of protein. Each bar is the average of three determinations, and the error bars are the standard deviations of the mean (n=3).