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. Author manuscript; available in PMC: 2010 Sep 20.
Published in final edited form as: J Biol Chem. 2000 Nov 3;275(44):34681–34692. doi: 10.1074/jbc.M002003200

FIG. 5.

FIG. 5

The UV absorbance peaks for 0 µm (●), 25 µm (○), 50 µm (□), 75 µm (♦), and 100 µm (Δ) chloramine-T in 0.5 m sodium phosphate buffer ± 0.1 m NaCl at pH 7.2 (A) or pH 5.5 (C). One major peak at 219–224 nm characterized the absorbance for chloramine-T. The UV absorbance for 15 µm pyridoxamine reacted with increasing concentrations of chloramine-T ± 0.1 m NaCl for 15 min RT at pH 7.2 (B) or pH 5.5 (C). The UV absorbance scans for 15 µm vitamin B6 reacted with increasing concentrations of chloramine-T in 0.5 m sodium phosphate buffer pH 5.5 ± 0.1 m NaCl for 15 min at RT (D), and then incubated overnight (16–18 h) at 37 °C.