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. Author manuscript; available in PMC: 2010 Sep 20.
Published in final edited form as: Arzneimittelforschung. 2010;60(7):445–451. doi: 10.1055/s-0031-1296310

Figure 1. Angiotensin II and LDL stimulates mRNA expression of CYP11B2 in human primary mesangial cells.

Figure 1

A: Human primary mesangial cells were incubated for 4 hrs with 10−7 M of angiotensin II and/or 40 mg/dL cholesterol of LDL in the presence of 5.5 mM of glucose with an appropriate amounts of mannitol for adjusting the same osmolarity as 25.0 mM glucose, and mRNA levels of CYP11B2 were determined with RT-PCR. B: Human primary mesangial cells were incubated for 4 hrs with 5.5 mM or 25.0 mM of glucose with an appropriate amounts of mannitol for adjusting the same osmolarity as 25.0 mM glucose, and mRNA levels of CYP11B2 were determined with RT-PCR.

Bars represent mean ± SD values of relative mRNA expression of CYP11B2 shown as fold induction to the baseline obtained in three different experiments. Each experiment was performed as triplicate.

a: p < 0.05, b: p < 0.01, c: p < 0.001, compared to the conditions indicated.