Table 1.
Nucleotide | Formula | Molecular mass, Da | Mass shift, Da |
---|---|---|---|
dATP | C10H16N5O12P3 | 491.2 | 313.2 |
dTTP | C10H17N2O14P3 | 482.2 | 304.2 |
dCTP | C9H16N3O13P3 | 467.2 | 289.2 |
dGTP | C10H16N5O13P3 | 507.2 | 329.2 |
ddATP | C10H16N5O11P3 | 475.2 | 297.2 |
ddTTP | C10H17N2O13P3 | 466.1 | 288.2 |
ddCTP | C9H16N3012P3 | 451.1 | 273.2 |
ddGTP | C10H16N5O12P3 | 491.2 | 313.2 |
Chemical composition of deoxy- as well as dideoxynucleotides and their molecular masses. The value for the mass shift refers to the respective increase in molecular mass when incorporated into a single stranded oli-gonucleotide.