TRAIL induces phosphorylation and mitochondrial translocation of PKCδ that is dependent on activity of phospholipases. HeLa cells were either untreated or preincubated with D609 and R59949 (50 μmol/L) for 30 minutes and subsequently treated with TRAIL (10 ng/mL) for the indicated times (A and B) or for 6 hours (C). Whole cell lysates (A) and mitochondrial fractions (B and C) were analyzed by immunoblotting using antibodies specific for phosphorylated PKCδ, whole PKCδ, actin, and HSP60. The band intensities of phospho-PKCδ (Ser643), phospho-PKCδ (Thr505), and PKCδ were analyzed with a densitometer using data from three independent experiments (bottom). **, 0.05 < P < 0.10, statistically significant two-sided Wilcoxon test compared with untreated samples.