Table 1.

Fidelity of pol δ.

Base pair	kcat	Km	kcat/Km	Error frequencya
	min−1	μM	μM−1min−1
dGTP·G	0.020 ± 0.004	630 ± 350	3.2 × 10−5	1.6 × 10−5
dATP·G	0.020 ± 0.002	380 ± 150	5.3 × 10−5	2.7 × 10−5
dTTP·G	0.062 ± 0.001	400 ± 30	1.6 × 10−4	8.0 × 10−5
dCTP·G	2.7 ± 0.4	1.4 ± 0.6	1.9	N/Ab
dGTP·A	0.024 ± 0.006	780 ± 440	3.1 × 10−5	5.2 × 10−5
dATP·A	0.044 ± 0.008	960 ± 360	4.6 × 10−5	7.8 × 10−5
dTTP·A	3.9 ± 1.0	6.6 ± 3.0	0.59	N/Ab
dCTP·A	0.020 ± 0.002	500 ± 170	4.0 × 10−5	6.8 × 10−5
dGTP·T	0.10 ± 0.01	210 ± 20	4.8 × 10−4	4.5 × 10−4
dATP·T	2.2 ± 0.2	2.1 ± 0.6	1.0	N/Ab
dTTP·T	0.022 ± 0.001	410 ± 70	5.4 × 10−5	5.1 × 10−5
dCTP·T	0.045 ± 0.002	410 ± 70	1.1 × 10−4	1.0 × 10−4
dGTP·C	3.8 ± 0.4	2.5 ± 0.8	1.5	N/Ab
dATP·C	0.042 ± 0.004	250 ± 100	1.7 × 10−4	1.1 × 10−4
dTTP·C	0.11 ± 0.01	660 ± 160	1.7 × 10−4	1.1 × 10−4
dCTP·C	N/Dc	N/Dc	< 2 × 10−5	< 1 × 10−5

^aThe error frequency is the ratio of the k_cat/K_m parameter for incorporating the incorrect dNTP to the sum of the k_cat/K_m parameters for incorporating the correct and the three incorrect dNTPs.

^bNot applicable

^cNot detectible.