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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Aug 5;30(10):1976–1982. doi: 10.1161/ATVBAHA.110.212415

Figure 6. Role of GIT1 tyrosine phosphorylation in PDBU-induced ERK1/2 activation.

Figure 6

HEK 293 cells were transfected with (A) pcDNA3, Flag-GIT1 (WT), Flag-GIT1 (Y293F), or Flag-GIT1 (Y392F) or (B) pcDNA3, Xpress-GIT1 (WT), or Xpress-GIT1 (Y321F) for 24 hours and starved for 6 hours. After treatment with or without 1μM PDBU for 10 min, cell lysates were immunoblotted with specific pERK1/2 antibody (upper panels) and reprobed with ERK2 antibody (lower panels). Flag (or Xpress) antibody blot shows the expression of various GIT1 plasmids, and β-actin antibody blot shows equal loading. The blots were analyzed by densitometry using LiCor software. Fold changes normalized to the first lane are shown below the blots (n=2-3).