Table 3.
Infection of Human Liver–Chimeric Mice with Hepatitis C Virus (HCV) Genotypes 1–6 Strains from Titrated Challenge Pools
| HCV genotype pool inocula | Plasma HCV RNA titer by week, log10 IU/mL | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| uPA-SCID mouse ID | Plasma human albumin level at week 0, mg/mL | Genotype | Strain | Dose,a log10 CID | Dose,a log10 IU | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Test used |
| K202 | 5.2 | 1a | H77C | 2.6 | 3.9 | NA | 7.7 | NA | NA | NA | NA | NA | NA | T |
| K230 | 4.0 | 1a | H77C | 2.6 | 3.9 | NA | 7.7 | NA | NA | NA | NA | NA | NA | T |
| K292 | 2.2 | 1a | H77C | 2.6 | 3.9 | NA | 7.8 | NA | NA | NA | NA | NA | NA | T |
| K292R | 2.7 | 1a | H77C | 2.6 | 3.9 | NA | 7.6 | NA | NA | NA | NA | NA | NA | T |
| K16R | 2.1 | 1b | HC-J4 | 1.3 | 3.0 | NA | 6.4 | NA | 7.9 | NA | 7.5 | NA | NA | M |
| K21L | 7.4 | 1b | HC-J4 | 1.7 | 3.4 | NA | >2.7 | NA | NA | NA | NA | NA | NA | M |
| K7 | 2.8 | 1b | HC-J4 | 2.0 | 3.7 | NA | >2.7 | NA | NA | NA | 7.9 | NA | NA | M |
| K7L | 3.0 | 1b | HC-J4 | 2.0 | 3.7 | NA | >2.7 | NA | NA | NA | 7.9 | NA | 7.2 | M |
| K179-L | 4.7 | 2a | HC-J6 | 3.0 | 4.0 | NA | NA | 7.4 | NA | 6.5 | NA | 5.9 | 4.9 | M |
| K637 | 2.1 | 2b | HC-J8 | 3.0 | 3.6 | <2.9 | +b | 4.1 | 5.3 | 5.6 | 5.3 | 5.1 | NA | T |
| K595 | 6.4 | 3a | S52 | 2.0 | 3.3 | 4.6 | 6.0 | 6.6 | 6.5 | 6.6 | 6.2 | 5.9 | NA | T |
| K328-R | 6.2 | 4a | ED43 | 3.6 | 4.2 | NA | 7.4 | NA | + | + | NA | NA | NA | T |
| K698 | 2.6 | 5a | SA13 | 3.0 | 4.1 | +b | 4.5 | 4.8 | 5.4 | NA | NA | NA | NA | T |
| K686 | 4.6 | 6a | HK-6a | 2.0 | 3.9 | NA | 4.0 | 6.2 | 7.1 | 7.2 | NA | NA | NA | T |
NOTE. Plus signs indicate unquantified positive detections of HCV RNA. Uninfected mice are not shown (see text). CID, chimpanzee infectious dose; M, Roche HCV Monitor 2.0 assay; NA, not available; T, Roche HCV TaqMan48 assay; uPA-SCID, urokinase-type plasminogen activator–severe combined immunodeficient.
a
The titer in CIDs is the titer listed in Table 2, corrected to reflect the relative amount of the inoculum.
b
HCV RNA level, <1500 IU/mL.