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. Author manuscript; available in PMC: 2011 Jul 30.
Published in final edited form as: Brain Res. 2010 May 16;1346:1–13. doi: 10.1016/j.brainres.2010.05.030

Figure 8. GABA promotes surface expression of GABAA receptors composed of α1β2 and α1β2(LL/AA) subunits.

Figure 8

HEK 293 cells were transfected with α1β2V5 or α1β2V5(LL/AA) subunit cDNAs. Cells were incubated throughout the transfection and expression period in the absence or presence of 100 μM GABA. Forty-two hrs post-transfection, cell surface and intracellular GABAA receptor populations were labeled by indirect immunofluorescence using an anti-V5 antibody and imaged by confocal microscopy. Images representative of at least 5 independent experiments each performed in triplicate.