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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Vaccine. 2010 Aug 8;28(41):6714–6722. doi: 10.1016/j.vaccine.2010.07.084

Figure 2. Evaluation of protein hydrophobicity and export.

Figure 2

(A) Hydropathy plot was performed for Lcrv and ssLcrV antigens, based on the parameters proposed by Kyte & Doolittle. (B) Wildtype LcrV- and ssLcrV-expressing L. plantarum were disrupted with a French® press, the insoluble material (cell envelope) was extracted with Triton X-114 and partitioned into detergent and aqueous phases. Protein fractions were analyzed on a SDS-PAGE and tested by immunoblot with LcrV-specific monoclonal antibody 40.1. (C) Protein was quantified by densitometry. The results were plotted as a percentage of the total LcrV content for each recombinant Lactobacillus. TE: total extract; D: detergent phase; A: aqueous phase; wild type LcrV-, and ssLcrV-expressing L. plantarum (LpV, and LpssV, respectively). *p=0.003; ns: not significant.